Jun Bai

Abstract

Background: In order to shorten drug’s evolution time than personal cancer’s evolution time, a Tag-Tack platform was designed and constructed to make introcytoplastic missense proteins to be druggable in 6 months.

Methods: We screened 8 human ScFVs for 6 introcytoplastic missense proteins TP53 p.R248Q, KRAS p. G13D, BRAF p.V600E, IDH1 p.R132C, PIK3CA p.E545K, EML4-ALK v3a fusion, and cloned them into the platform to be TagTacker.

Results: 6 cell lines harboring the former 6 introcytoplastic missense proteins were target killed by those 8 Tag-Tackers, while no tagging protein cell-lines were alive. Based on those in vtro cell line’s results, an out of treatment patient having BRAF p.K601E mutation was successfully treatmented by lesion injection of BRAF p.K601E Tagtacker’s Compassionate using.

Conclusions: Tag tack, a single amino acid resolution anti tumor platform which can make any introcytoplastic missense proteins to be druggable in 6 months is standby!

Audience take away:

1. Any introcytoplastic proteins can be druggable at Tag-tack platform!

2. This draggable time can be shortened less than 6 mouths!

3. Any cancer patient can get personal Tag-tacker drugs in 6 mouths at Tag-tack platform!